The department of laboratory Medicine has been the back bone for the diagnosis and therapy monitoring for the patients. The department has a menu of 450 tests and for ease and better growth of the specialties the department is structured as below.

The department has a team of 50 qualified and skillful paramedical staff to help in the technical activities. The department offers the diagnostic facility not only our OPD but also to other health care centres. To cater the diagnostic services further, satellite collection centres are initiated to provide help for the patients in adjoining areas of Indore and various parts of the State. Home sample collection facility is also provided for the convenience of the patients (call Mr. Jitendra Joshi : 9826511348)

Quality Control :-
To ensure quality for the vast menu of the analytes, excellent quality reagents and superb equipments are used by the dedicated technical experts. To avoid pre-analytical errors, blood samples are collected by skilled technicians using internationally acclaimed vaccutainer system. There is participation in national and international quality control programmes . Internal and external quality control samples are run regularly to monitor the quality. With the quality control measures was used, medical fraternity of M.P. confides in the test reports of the department.

Hematology :-
Coulter HMX Transasia XT-1800i (5 Part differential counter) and Sysmax KX21 ( 3 Part differential counter) are the working horses for Hematology. The department participates regularly in International QC programme EQUAS and scores high ranks. Coagulation studies are carried out using Stago Analyzer and Stago QC controls are run daily to have QC checks. Two clinical Onco hematologists besides the two Pathologists give continuous technical support to have reputed hematology services. During the Dengue out break, hematology, Blood Banking together with Microbiology played a key role in providing diagnostic and therapeutic monitoring facility for the city.

Clinical Pathology :-
Roche urine analyzer is added up recently to have faster and precise results. The unit analyses over 100 samples daily (Urine, stool, body fluid etc.).

Blood Bank :-
The blood bank collects over 800 units of blood every month from voluntary and relative donors and offers an array of blood components for optimal utilization of the precious biological material. The blood Bank has a huge capacity of storage of blood and components in the form of walk in cooler and – 30 0C deep freeze .The facilities is used not only by CHRC but also by several nursing homes and hospitals in the city.

The components are given at subsidized rates and include FFP, platelet rich plasma and packed RBCs.

We boast of our stringent screening of blood units for HIV, HBsAg, HCV and RPR.

Biochemistry :-
The Biochemistry Division is equipped with fully automated equipments such as Hitachi 704, MTA 500 blood gas analyzer, Easy-lyte electrolyte analyzer and chromatography facility.

Recently we have also acquired two Emola (Randox access Biochemistry analyzer), ABL 555 blood gas analyzer, densitometer (RAL) for Quantitative protein and hemoglobin electrophoresis. Spectrophotometer (Thermo) and sweat chloride analysis has also been added.

The laboratory participates in the national and international quality control programmes and attains high scores, which speaks of its quality.

The biochemistry also has facility of therapeutic drug analysis.

Histopathology :-
Histopathology unit has creditable facility of frozen section cutting besides the routine biopsy processing. Cytology of cervical smears, sputum and of all body fluids is done. This is reputed department for reporting on fine needle aspiration. Immune-marker studies will be added shortly, along with marker studies like ER-PR for Breast cancers.

Microbiology, Immunology and Molecular biology Division

Microbiology Lab :-
The Microbiology lab holds an outstanding reputation throughout India. It provides facility for aerobic, anaerobic and fungal cultures. The Bactec 9050 automated system for Blood Culture has been a boon for the entire city.

It also provides bacteriological analysis of water, food analysis, sterility testing, Disinfectants and anti-microbial testing facility. The lab is perhaps the only lab making autovaccine (for recurrent throat and respiratory infections, oral ulcers, nasal polyps ,acne, asthma, recurrent boils and warts) in India and large number of patients have been benefited with our autovaccine. The lab also prepares autovaccine for treating viral warts.

The department has done extensive epidemiological work on the infectious diseases like tuberculosis, typhoid, viral hepatitis, malaria, chikunguniya, dengue, leptospirosis, HIV etc and provided valuable information to State health authorities.

The department has participated in several international Programmes on the surveillance of bacterial drug resistance and performs weekly Q.C. using CLSI standards.

Ministry of Health and family welfare in the process of establishing / strengthening public health laboratories has sought expertise from our Microbiology Dept.

HLA Lab: With the renal transplant facility at CHRC,HLA lab was set up in 1987 and HLA typing (ABC, DR) and cross matching is being done since then. HLA B27 has been a popular test at our end and samples are received from various parts of the state.

Mycobacteriology Lab :-
The laboratory has established the entire array of investigations for the diagnosis of tuberculosis including detection and differentiation of M. tuberculosis and MOTT by Real Time PCR, T-Cell interferon tests. The growing need of tuberculosis forced us to develop a separate Mycobacteriology lab.

The labis accridiated as Intermediate Referral Lab (IRL) by RNTCP for Culture and Drug sensitivity testing in M.P. State. Dr. Nanda Hemvani, working on research programme has designed and developed a phage –mediated assay for the drug susceptibility testing of TB bacilli from direct clinical samples and the new method can yield results in 48-72 hrs. against the conventional methods which take 6-8 weeks. The new method is expected to revolutionize the drug sensitivity reporting for TB patients.

Molecular Biology :-
PCR technique was introduced way back in 1995 in the lab and with the introduction of Real Time PCR System, Rotor gene 3000, Corbett Research; Australia in 2006, the full pledged molecular biology has evolved. The viral loads / detection is done for HIV, HBV, HCV, Dengue, HSV, JE, Chikunguniya, Influenza A, TB, Bird flue etc. Shortly the facility for thalessaemia gene mutation, cancer detection, drug resistant detection will be added.

Immunology :-
The unit boasts of the immunofluorescent technique for ANF, Auto-immune Liver diseases, Atypical Pneumonias, Renal Immunofluorescence, Platelet antibodies etc and is the only lab having immunofluorescent technique facility in M.P. State.

The antibody /antigen tests are being carried out for all the infectious diseases including TORCH, Herpes, Measles, mumps, amoebiosis, dengue, leptospira and several others. The lab has an entire gamut of markers for viral hepatitis and has carried out extensive epidemiological work in the area. The cancer markers, hormones, metabolites like homocystine, B-12, Ferritin, cardiac markers and other diagnostic analytes are assayed using three efficient automated systems namely AIA-600 (Tosho, Japan), Architect (Abbott), Cobas e 411 (Roche). The lab participates in international EQUAS QC Programme. Immunoglobulin assay, RA, ASO, CRP, Complement, micro albumin assay are performed using immunoturbidometry.

New tests being continuously added for improving the diseases diagnosis e.g. BNP, Anti CCP, ASCA, Allergen testing were added to the list in 2010. Microbiology gives help not only in the diagnosis but has given a pivotal support in Infection Control and BMW management programme.

It is worth to mention that surveillance programme conducted by Microbiology lab on infectious diseases such as malaria, dengue, typhoid, and cholera gives valuable information to the local health authorities and helped to control the out breaks.

Academic Programme

Ph.D. Programme :
Department runs Ph.D. Microbiology Programme affiliated to Devi Ahilya University. Over 140 research papers in National and International journals have been published from the department (Publications Research) and bagged several awards for research work.

Training /dissertation programme :
The Microbiology lab offers training /dissertation programme for M. Sc. students (IV Semester) and students from M.P. State and other states compete to seek admission for the programme.

The dept has several on going research projects supported by ICMR, DST and other funding agencies. (Research).

The department also imparts training to CMLT and DMLT students of Choithram Institute of Paramedical Sciences affiliated to Devi Ahilya University.

Conferences and Workshop

Organization of Workshop and Conferences :
  • 7th Annual conference of Hospital Infection Society - 1997
  • National workshop on Biomedical waste Management -2004
  • CME and workshop on “recent trends in diagnosis of tuberculosis” at 54th Annual conference of Indian association of pathologists & microbiologists 2005
Research Areas
  • Tuberculosis
  • HIV
  • Viral Hepatitis
  • Malaria
  • Hospital Infection Control
  • Bio-medical waste and Environmental Research
  • Low level Lasers
  • Epidemiological study of Infectious diseases
  • Bacterial Drug Resistance, Evolution of MDR in community
  • Design and development of diagnostic tools
  • Atomic Force Microscopy
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